mouse anti human tnfr1 pe  (R&D Systems)

Journal: Cell Reports Medicine

Article Title: Personalized drug screening using patient-derived organoid and its clinical relevance in gastric cancer

doi: 10.1016/j.xcrm.2024.101627

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Article Snippet: The cells were then filtered with 70 μm cell strainers and stained with BUV395 Mouse Anti-Human CD3 (BD Biosciences), BV510 Mouse Anti-Human CD45 (BD Biosciences), APC Mouse Anti-Human CD8 (BD Biosciences), PE-CY7 Mouse Anti-Human TNF-α (BD Biosciences), BV605 Mouse Anti-Human IFN-γ (BD Biosciences).

Techniques: Recombinant, Staining, TUNEL Assay, Software

Journal: iScience

Article Title: Oleic acid triggers metabolic rewiring of T cells poising them for T helper 9 differentiation

doi: 10.1016/j.isci.2024.109496

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Article Snippet: Anti-human TNF; PE-Cy™7; Isotype Mouse; Clone MAb11 , BD Biosciences , Cat#557647; RRID: AB_396764.

Techniques: Activation Assay, Isolation, Recombinant, Modification, Staining, Expressing, Software

Journal: Frontiers in Immunology

Article Title: Isolation and expansion of pure and functional γδ T cells

doi: 10.3389/fimmu.2024.1336870

Figure Lengend Snippet: Antibodies.

Article Snippet: Mouse anti human TNF-α Pe-Cy7 , BD , 557647 , 1:100.

Techniques:

Journal: Frontiers in Immunology

Article Title: Isolation and expansion of pure and functional γδ T cells

doi: 10.3389/fimmu.2024.1336870

Figure Lengend Snippet: Effector molecule production and anti-tumor function of expanded Vδ1 + and Vδ2 + T cells. Expanded cells were stimulated with PMA/Ionomycin to assess their potential to produce effector molecules IFN-γ, TNF-α and granzyme (B) Tumor killing capacity was assessed with a coculture of freshly expanded γδ T cells with target cells lines WiDr, WM9, HAP1 and HT29. (A) Representative flow cytometry plots showing production of IFN-γ and TNF-α by Vδ1 + and Vδ2 + T cells stimulated with PMA/Ionomycin for 1.5 hour or unstimulated. (B–E) Summary of the production of IFN-γ and TNF-α by Vδ1 + and Vδ2 + T cells stimulated with PMA/Ionomycin for 1.5 hour or unstimulated. The percentage and MFI of the cytokine positive cells is depicted (F) Representative flow cytometry plots showing the production of granzyme B by Vδ1 + and Vδ2 + T cells stimulated with PMA/Ionomycin for 1.5 hour or unstimulated. (G, H) Summary of the production of granzyme B by Vδ1 + and Vδ2 + T cells stimulated with PMA/Ionomycin for 1.5 hour or unstimulated. The percentage and MFI of the granzyme B positive cells is depicted. (I) Representative flow cytometry plots showing the frequency of dead (NEAR-IR+) WiDr, WM9, HAP1 and HT29 target cells ( left column ) and target cells cocultured with Vδ1 + ( middle column ) or Vδ2 + ( right column ) T cells. (J–M) Summary of the percentage of dead target cells, WiDr, WM9, HAP1 and HT29, only (-) or with Vδ1 + or Vδ2 + T cells. Data are shown as the mean and standard deviation of four donors and the data of each donor represents the mean of triplicates. Data were analyzed by a one-way ANOVA followed by Tukey’s multiple comparisons test.

Article Snippet: Mouse anti human TNF-α Pe-Cy7 , BD , 557647 , 1:100.

Techniques: Flow Cytometry, Standard Deviation

Journal: BMC Immunology

Article Title: HIV infection and aging: enhanced Interferon- and Tumor Necrosis Factor-alpha production by the CD8 + CD28 - T subset

doi: 10.1186/1471-2172-2-10

Figure Lengend Snippet: The distribution of CD4 + and CD4 + T cells are shown for typical samples of PBMC from HIV, aged and normal (control) donors as evaluated by flow cytometry. The cells positive for IFN-γ (PE) or TNF-α (PE) distribute vertically above the horizontal marker; the vertical distance is a relative measure of cytokine intensity. The two populations of cells that comprise the CD3 positive population are the CD8 + T cells and the CD4 + T cells (CD3 + , CD8 - ) as shown on the X axis. The percentage of CD4 + or CD8 + T cells which contain cytokine are shown in the upper quadrants. The PBMC were activated for 15 hrs with anti-CD3 and PMA and subsequently treated as described in METHODS. In all cases, nonactivated controls (not shown) were examined; less than 0.5% of cells were cytokine positive.

Article Snippet: To the cell pellet, 3 ul of phycoerythrin (PE) conjugated mouse anti-human IFN-γ or TNF-α monoclonal Ab (Pharmingen Inc.) was added and incubated 30 min at 4°.

Techniques: Flow Cytometry, Marker

Journal: BMC Immunology

Article Title: HIV infection and aging: enhanced Interferon- and Tumor Necrosis Factor-alpha production by the CD8 + CD28 - T subset

doi: 10.1186/1471-2172-2-10

Figure Lengend Snippet: Secreted IFN-γ and TNF-α from T Cell Culture *

Article Snippet: To the cell pellet, 3 ul of phycoerythrin (PE) conjugated mouse anti-human IFN-γ or TNF-α monoclonal Ab (Pharmingen Inc.) was added and incubated 30 min at 4°.

Techniques: Cell Culture

Journal: BMC Immunology

Article Title: HIV infection and aging: enhanced Interferon- and Tumor Necrosis Factor-alpha production by the CD8 + CD28 - T subset

doi: 10.1186/1471-2172-2-10

Figure Lengend Snippet: The distribution of CD8 + T cells are shown as obtained from PBMC by four color flow cytometry using anti-CD28-PE and anti-IFN-γ-FITC (or anti-TNF-α-FITC) as described in METHODS. The CD28 + and CD28 - cells distribute vertically and the IFN-γ or TNF-α positive cells distribute horizontally according to their intensity. The percentage of the CD8 + CD28 + or CD8 + CD28 - T cells which contains cytokine are shown in the right quadrants. Cells were activated with anti-CD3 and PMA as described in METHODS. The controls refers to cells from a normal young donor. The HIV + and aged samples were derived from typical donors.

Article Snippet: To the cell pellet, 3 ul of phycoerythrin (PE) conjugated mouse anti-human IFN-γ or TNF-α monoclonal Ab (Pharmingen Inc.) was added and incubated 30 min at 4°.

Techniques: Flow Cytometry, Derivative Assay

Journal: BMC Immunology

Article Title: HIV infection and aging: enhanced Interferon- and Tumor Necrosis Factor-alpha production by the CD8 + CD28 - T subset

doi: 10.1186/1471-2172-2-10

Figure Lengend Snippet: The production of cytokine mRNA determined by quantitative by PCR is shown for (A) HIV + donor CD8 + T cells and (B) aged donor CD8 + T cells. The cDNA copies per ug RNA from the CD8 + T cells. The cDNA copies per ug RNA from the CD8 + T cells were derived from: 1, nonativated controls; 2, activated controls (INF-γ); 3, activated HIV + or aged (INF-γ); 4, activated controls (TNF-α); 5, HIV + or aged (TNF-α).

Article Snippet: To the cell pellet, 3 ul of phycoerythrin (PE) conjugated mouse anti-human IFN-γ or TNF-α monoclonal Ab (Pharmingen Inc.) was added and incubated 30 min at 4°.

Techniques: Real-time Polymerase Chain Reaction, Derivative Assay

Journal: BMC Immunology

Article Title: HIV infection and aging: enhanced Interferon- and Tumor Necrosis Factor-alpha production by the CD8 + CD28 - T subset

doi: 10.1186/1471-2172-2-10

Figure Lengend Snippet: Comparison of cytokine Production in CD8 + CD28 and CD8 + CD28 + T cells

Article Snippet: To the cell pellet, 3 ul of phycoerythrin (PE) conjugated mouse anti-human IFN-γ or TNF-α monoclonal Ab (Pharmingen Inc.) was added and incubated 30 min at 4°.

Techniques:

Journal: Cell Reports

Article Title: Broadly recognized, cross-reactive SARS-CoV-2 CD4 T cell epitopes are highly conserved across human coronaviruses and presented by common HLA alleles

doi: 10.1016/j.celrep.2022.110952

Figure Lengend Snippet: Functional characterization of in vitro -expanded cross-reactive T cells (A) Gating. (B) Representative plot of CD4/CD8 distribution in the CD3 + population and percentage of CD4 + cells (bar graph) in cells expanded from 3 donors. (C) Representative plot of CD45RA/CD197 in the CD4 + population and summary of the percentage of naive (N), central memory (CM), effector memory (EM), and EM re-expressing RA (TEMRA) populations in expanded cells (n = 3). (D) Representative ICS plots for IFN-γ, TNF-α, and IL-2 production, and CD107a mobilization, in the CD3 + population after re-stimulation of expanded cells with SARS-CoV-2 S pool (CoV-2 S) or peptides S 811–826 and S 816–831 ; positive responses shown in red boxes (>3-fold background). (E) Visualization of the polyfunctional response using Simplified Presentation of Incredibly Complex Evaluations (SPICE) ( Roederer et al., 2011 ): bar graph (means ± standard deviations) for each stimulating antigen (red for CoV-2, light green for S 811–826 , dark green for S 816–831 ) and comparison to control (gray) (p < 0.05, Wilcoxon rank-sum test); pie and arcs show the combined contribution of each marker (pie slice colors correspond to colors shown at the bottom of the bar graphs). (F) t-SNE analysis of concatenated data from 3 donors for stimulation with same antigens, showing density plots for each condition. Two gates (g1 and g2) were drawn, indicating major differences among stimulated and unstimulated samples. Histograms show IFN-γ, TNF-α, and CD107a in each gate. Representative density plots for responses of d0801 are shown (see also Figure S2 ).

Article Snippet: mouse anti-human TNF-α-PE-Cy7 (MAb11) , BD Biosciences , 557647.

Techniques: Functional Assay, In Vitro, Expressing, Marker

Journal: Cell Reports

Article Title: Broadly recognized, cross-reactive SARS-CoV-2 CD4 T cell epitopes are highly conserved across human coronaviruses and presented by common HLA alleles

doi: 10.1016/j.celrep.2022.110952

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Article Snippet: mouse anti-human TNF-α-PE-Cy7 (MAb11) , BD Biosciences , 557647.

Techniques: Produced, Recombinant, Staining, Gel Extraction, Enzyme-linked Immunospot, Enzyme-linked Immunosorbent Assay, Software, Binding Assay, Flow Cytometry, Sequencing, Filtration